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rabbit polyclonal anti tgn 46 antibody  (Novus Biologicals)
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Rabbit Polyclonal Anti Tgn 46 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Immunofluorescence microscopy displaying localization of PLD4 (red) at <t>TGN46-tagged</t> trans Golgi networks (green) in resting B cells and translocation of PLD4 from trans Golgi networks in CpG/anti-IgG + M stimulated B cells, compared to the control cells. (B) Immunofluorescence microscopy shows enhanced expression of PLD4 (red) and co-localization of PLD4 and CD63 (green) in CpG/anti-IgG + M stimulated B cells, compared to the control cells. Cell membranes stained with MemBrite appeared thicker in CpG/anti-IgG + M stimulated B cells than in the control cells. (C) Statistical analysis revealing enhanced PLD4 expression in each CpG/anti-IgG + M stimulated B cell compared to the control cells. Bar; 10 μm, **P < 0.01, ***P < 0.001.
Rabbit Anti Tgn46 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Immunofluorescence microscopy displaying localization of PLD4 (red) at <t>TGN46-tagged</t> trans Golgi networks (green) in resting B cells and translocation of PLD4 from trans Golgi networks in CpG/anti-IgG + M stimulated B cells, compared to the control cells. (B) Immunofluorescence microscopy shows enhanced expression of PLD4 (red) and co-localization of PLD4 and CD63 (green) in CpG/anti-IgG + M stimulated B cells, compared to the control cells. Cell membranes stained with MemBrite appeared thicker in CpG/anti-IgG + M stimulated B cells than in the control cells. (C) Statistical analysis revealing enhanced PLD4 expression in each CpG/anti-IgG + M stimulated B cell compared to the control cells. Bar; 10 μm, **P < 0.01, ***P < 0.001.
Rabbit Anti Tgn46 Polyclonal Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Immunofluorescence microscopy displaying localization of PLD4 (red) at <t>TGN46-tagged</t> trans Golgi networks (green) in resting B cells and translocation of PLD4 from trans Golgi networks in CpG/anti-IgG + M stimulated B cells, compared to the control cells. (B) Immunofluorescence microscopy shows enhanced expression of PLD4 (red) and co-localization of PLD4 and CD63 (green) in CpG/anti-IgG + M stimulated B cells, compared to the control cells. Cell membranes stained with MemBrite appeared thicker in CpG/anti-IgG + M stimulated B cells than in the control cells. (C) Statistical analysis revealing enhanced PLD4 expression in each CpG/anti-IgG + M stimulated B cell compared to the control cells. Bar; 10 μm, **P < 0.01, ***P < 0.001.
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(A) Immunofluorescence microscopy displaying localization of PLD4 (red) at <t>TGN46-tagged</t> trans Golgi networks (green) in resting B cells and translocation of PLD4 from trans Golgi networks in CpG/anti-IgG + M stimulated B cells, compared to the control cells. (B) Immunofluorescence microscopy shows enhanced expression of PLD4 (red) and co-localization of PLD4 and CD63 (green) in CpG/anti-IgG + M stimulated B cells, compared to the control cells. Cell membranes stained with MemBrite appeared thicker in CpG/anti-IgG + M stimulated B cells than in the control cells. (C) Statistical analysis revealing enhanced PLD4 expression in each CpG/anti-IgG + M stimulated B cell compared to the control cells. Bar; 10 μm, **P < 0.01, ***P < 0.001.
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polyclonal rabbit anti tgn46  (Proteintech)
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(A) Immunofluorescence microscopy displaying localization of PLD4 (red) at <t>TGN46-tagged</t> trans Golgi networks (green) in resting B cells and translocation of PLD4 from trans Golgi networks in CpG/anti-IgG + M stimulated B cells, compared to the control cells. (B) Immunofluorescence microscopy shows enhanced expression of PLD4 (red) and co-localization of PLD4 and CD63 (green) in CpG/anti-IgG + M stimulated B cells, compared to the control cells. Cell membranes stained with MemBrite appeared thicker in CpG/anti-IgG + M stimulated B cells than in the control cells. (C) Statistical analysis revealing enhanced PLD4 expression in each CpG/anti-IgG + M stimulated B cell compared to the control cells. Bar; 10 μm, **P < 0.01, ***P < 0.001.
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(A) Immunofluorescence microscopy displaying localization of PLD4 (red) at <t>TGN46-tagged</t> trans Golgi networks (green) in resting B cells and translocation of PLD4 from trans Golgi networks in CpG/anti-IgG + M stimulated B cells, compared to the control cells. (B) Immunofluorescence microscopy shows enhanced expression of PLD4 (red) and co-localization of PLD4 and CD63 (green) in CpG/anti-IgG + M stimulated B cells, compared to the control cells. Cell membranes stained with MemBrite appeared thicker in CpG/anti-IgG + M stimulated B cells than in the control cells. (C) Statistical analysis revealing enhanced PLD4 expression in each CpG/anti-IgG + M stimulated B cell compared to the control cells. Bar; 10 μm, **P < 0.01, ***P < 0.001.
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rabbit polyclonal antibody against tgn46  (Novus Biologicals)
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Novus Biologicals rabbit polyclonal antibody against tgn46
(A) Immunofluorescence microscopy displaying localization of PLD4 (red) at <t>TGN46-tagged</t> trans Golgi networks (green) in resting B cells and translocation of PLD4 from trans Golgi networks in CpG/anti-IgG + M stimulated B cells, compared to the control cells. (B) Immunofluorescence microscopy shows enhanced expression of PLD4 (red) and co-localization of PLD4 and CD63 (green) in CpG/anti-IgG + M stimulated B cells, compared to the control cells. Cell membranes stained with MemBrite appeared thicker in CpG/anti-IgG + M stimulated B cells than in the control cells. (C) Statistical analysis revealing enhanced PLD4 expression in each CpG/anti-IgG + M stimulated B cell compared to the control cells. Bar; 10 μm, **P < 0.01, ***P < 0.001.
Rabbit Polyclonal Antibody Against Tgn46, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Immunofluorescence microscopy displaying localization of PLD4 (red) at TGN46-tagged trans Golgi networks (green) in resting B cells and translocation of PLD4 from trans Golgi networks in CpG/anti-IgG + M stimulated B cells, compared to the control cells. (B) Immunofluorescence microscopy shows enhanced expression of PLD4 (red) and co-localization of PLD4 and CD63 (green) in CpG/anti-IgG + M stimulated B cells, compared to the control cells. Cell membranes stained with MemBrite appeared thicker in CpG/anti-IgG + M stimulated B cells than in the control cells. (C) Statistical analysis revealing enhanced PLD4 expression in each CpG/anti-IgG + M stimulated B cell compared to the control cells. Bar; 10 μm, **P < 0.01, ***P < 0.001.

Journal: PLOS One

Article Title: Activated human B cells produce phospholipase D4-containing extracellular vesicles

doi: 10.1371/journal.pone.0329832

Figure Lengend Snippet: (A) Immunofluorescence microscopy displaying localization of PLD4 (red) at TGN46-tagged trans Golgi networks (green) in resting B cells and translocation of PLD4 from trans Golgi networks in CpG/anti-IgG + M stimulated B cells, compared to the control cells. (B) Immunofluorescence microscopy shows enhanced expression of PLD4 (red) and co-localization of PLD4 and CD63 (green) in CpG/anti-IgG + M stimulated B cells, compared to the control cells. Cell membranes stained with MemBrite appeared thicker in CpG/anti-IgG + M stimulated B cells than in the control cells. (C) Statistical analysis revealing enhanced PLD4 expression in each CpG/anti-IgG + M stimulated B cell compared to the control cells. Bar; 10 μm, **P < 0.01, ***P < 0.001.

Article Snippet: The following antibodies were used: Recombinant human anti-human PLD4 therapeutic antibody biotinylated (TAB-759CL-Biotin; Creative Biolabs, NY, USA); rabbit anti-PLD4 polyclonal antibody (HPA051512; Atlas Antibodies, Bromma, Sweden); rat anti-CD9 monoclonal antibody (017−28211; FUJIFILM Wako, Osaka, Japan); FITC mouse anti-human CD63 antibody (353005; BioLegend, San Diego, CA, USA); PerCP/Cyanine5.5 mouse anti-human CD19 antibody (302230; BioLegend); Mouse anti-human CD19 antibody (302202; BioLegend); rabbit anti-TGN46 polyclonal antibody (13573–1-AP; Protein Tech, IL, USA).

Techniques: Immunofluorescence, Microscopy, Translocation Assay, Control, Expressing, Staining